Use | the treatment of acute high altitude hypoxia can achieve good results, through the antioxidant effect can significantly improve the damage to the brain tissue, and can reduce the level of serum malondialdehyde, improve the content of Serum Superoxide dismutase, has a high value in clinical treatment. for content determination/identification/pharmacological experiments, etc |
preparation | take about 2.5g of Rosa roxburghii Tratt powder, weigh it precisely, put it in a 250ml round-bottom flask, add 100ml of methanol, water Bath reflux, filtration, the residue was washed with 40ml methanol 4 times, each time 10ml, combined filtrate and concentrated to about 10ml, added to neutral alumina column (100~120 mesh, 10g, the eluent was collected and concentrated to dryness. The residue was dissolved in methanol and transferred to a 10ml measuring flask, diluted to the scale, and shaken, filter with a microporous filter (0.45 μm), and take the filtrate. At the same time, the experimental results of different reflux time extraction were investigated. Finally, the extraction method was determined to use methanol as solvent, reflux extraction for 1H, and then through the neutral alumina column, drying to obtain the rose. |